Figure 6From: Functional expression of a penicillin acylase from the extreme thermophile Thermus thermophilus HB27 in Escherichia coliSubcellular location of the quimeric protein Sp Eco -PAC Tth in Tth cells. The overexpression was performed in the Δ pac Tth mutant strain. Immunodetection of α- and β-PAC subunits was performed on the soluble protein fraction (SB) or in the membrane fraction (MB). Tth PAC matured in Tth cells was used as control (C).Back to article page