Figure 5From: Functional expression of a penicillin acylase from the extreme thermophile Thermus thermophilus HB27 in Escherichia coliTrypsin accessibility assays. Tth PAC α- and β-subunits were immunodetected in Tth NAR1 Δ slpA strain before trypsin treatment (C), and after a digestion with 1.2 mg/ml of trypsin (1) or with 5.5 mg/ml of trypsin (2). EDTA at 5 mM was used to get a partial solubilization of the outer membrane. Nqo1 was used as a cytoplasmic marker.Back to article page