Physical determinants of the wine yeast life span. The CLS analyses of strain EC1118 in SC medium were done. The assays were performed by making serial dilutions, plating an aliquot and counting colonies. CFU (colony-forming units)/mL were expressed in relation to the values obtained from the day-3 post-inoculation, thatwere considered to be 100% survival. A) Effect of temperature on CLS. B) Effect of hyperosmorality on CLS. C) Effect of pH on CLS. Experiments were done in triplicate, and the mean and standard deviation are provided.