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Figure 1 | Microbial Cell Factories

Figure 1

From: Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes

Figure 1

Change of the intracellular proteome of P. pastoris GS115 in response to methanol-induced high-level production of the Hepatitis B surface antigen. Sections of 2D gels representing parts of the intracellular proteome (A) at the end of the glycerol batch phase and (B) 114 hours after the onset of methanol feeding. For encircled protein spots (dark lines) time-dependent changes are given below. Magnified 2D gel sections from samples taken at the end of the glycerol batch phase and 4, 16, 72 and 114 hours after the onset of methanol feeding with representative proteins involved in (C) methanol metabolism and (D) oxidative and (E) ER stress responses (including also vacuolar degradation).

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Microbial Cell Factories

ISSN: 1475-2859

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