Table 4 Oligonucleotides utilized in this study
From: Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris
Primer | Oligonucleotide sequence |
|---|---|
BC-F | GCTGCTAAAGAAGAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
BC-R | GTAAGTGCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
A-F | GGCCGCGAATTAATTCGCCTTAGACATG |
A-R | AGATCTTGATATAAATTTCACGTTTAAAATC |
D-F | TCGACAATTGGTTTGACTAATTCCATAATCTG |
D-R | ACCTTTCGTCTTTGGATGTTAGTCT |
BC-F10 | ATTCCCTAGG |
BC-R10 | TCGCGGCCGC |
BC-F25 | TGAAGCTTACGTAGAATTCCCTAGG |
BC-R25 | CTAAGGCGAATTAATTCGCGGCCGC |
BC-F40 | CGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
BC-R40 | GAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
BC-F55 | GAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
BC-R55 | GCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
BC-F70 | CATTGCTGCTAAAGAAGAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
BC-R70 | CGGTCTTCTCGTAAGTGCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
BC-F85 | TACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
BC-R85 | AGAATCTAGCAAGACCGGTCTTCTCGTAAGTGCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |
BC-F65 | CTGCTAAAGAAGAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTTACGTAGAATTCCCTAGG |
BC-R65 | TTCTCGTAAGTGCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCGAATTAATTCGCGGCCGC |