Table 2 Thermostability of enzyme variants from each generation
From: Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris
Generation | Variant | t1/2(min, at 60°C) | Fold Improvement |
|---|---|---|---|
0 | parent | 4.0 | 1.0 |
1 | ep 1-1 | 9.8 | 2.4 |
| Â | ep 1-26 | 8.7 | 2.2 |
2 | ep 2-3 | 11.3 | 2.8 |
| Â | ep 2-4 | 21.7 | 5.4 |
| Â | ep 2-8 | 14.4 | 3.6 |
| Â | ep 2-12 | 13.5 | 3.4 |
3 | S 3-1 | 38.8 | 9.7 |
| Â | S 3-4 | 58.9 | 13.9 |
| Â | S 3-18 | 48.0 | 12.0 |
4 | S 4-3 | 184.0 | 46.0 |
| Â | S 4-13 | 168.3 | 42.1 |
| Â | S 4-20 | 159.5 | 40.0 |