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Table 1 The effects of the fragment molar ratio of B/C:A:D and the length of the overlapping sequences on recombination efficiency

From: Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris

Method

Recombination efficiencyc(× 105, recombinants /μg DNA)

Fragment B/C:A:D (molar ratio) a

10:1:1

2.30

 

5:1:1

1.80

 

3:1:1

1.20

 

1:1:1

1.05

Length of overlapping sequences (bp) b

10

0.10

 

25

0.80

 

40

1.25

 

55

1.75

 

70

2.32

 

85

2.35

  1. a The length of overlapping sequence was kept at 65 bp.
  2. b The fragment molar ratio of B/C:A:D was kept at 10:1:1.
  3. c Recombination efficiency is the number of recombinant cells (recombinants) generated by 1 μg of DNA fragments in a transformation procedure.
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Microbial Cell Factories

ISSN: 1475-2859

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