Adsorption of β-galactosidase of Alicyclobacillus acidocaldarius on wild type and mutants spores of Bacillus subtilis

Table 4 Synthetic oligonucleotides

Oligonucleotide	Sequence (5’- 3’)^a	Restriction site	Position of annealing^b
cotS3	ggatccATCGACCATGTGGCGCTG	Bam HI	+64/+82
cotS4	aagcttAACCATCACTTTATTCAG	Hind III	+771/+758
cotZ1	gcatgcGCTGTTGAAGAAGACTGC	Sph I	+64/81
cotZ2	gtcgacAACTTCAATACAGTAGTTCG	Sal I	+378/+359
cgeA1	gcatgcCAACTGCAACAGAAGGAG	Sph I	+64/+81
cgeA2	gtcgacGTGAACCTGATCGAAAGC	Sal I	+297/+279

^a Capital and lowercase letters indicate nucleotides complementary to corresponding gene DNA and unpaired flanking sequences carrying a restriction site, respectively. ^b Referred to cotS, cotZ or cgeA sequences, taking the first nucleotide of the initiation codon as +1.