Oligonucleotide | Sequence (5’- 3’)a | Restriction site | Position of annealingb |
---|---|---|---|
cotS3 | ggatccATCGACCATGTGGCGCTG | Bam HI | +64/+82 |
cotS4 | aagcttAACCATCACTTTATTCAG | Hind III | +771/+758 |
cotZ1 | gcatgcGCTGTTGAAGAAGACTGC | Sph I | +64/81 |
cotZ2 | gtcgacAACTTCAATACAGTAGTTCG | Sal I | +378/+359 |
cgeA1 | gcatgcCAACTGCAACAGAAGGAG | Sph I | +64/+81 |
cgeA2 | gtcgacGTGAACCTGATCGAAAGC | Sal I | +297/+279 |