Figure 1From: Adsorption of β-galactosidase of Alicyclobacillus acidocaldarius on wild type and mutants spores of Bacillus subtilisSchematic representation of a typical adsorption experiment. Purified spores were mixed to the purified enzyme in PBS buffer (pH 4.0) and incubated one hour at 25°C. The reaction was then stopped in ice and sample either directly assayed (Total Units) or fractionated by centrifugation and fractions assayed independently.Back to article page