Table 1 Bacterial strains and plasmids used in this study
Strain or plasmid | Genotype | Source or reference |
|---|---|---|
Strains | ||
Escherichia coli JM109 | Cloning host for pGEMTeasy- and pJRS233-derivative plasmids | Promega |
Lactobacillus reuteri 100-23 | Rodent isolate; wild type strain | [25] |
ΔgadB | Wild-type strain derivative with a deletion in gadB | This study |
Plasmids | ||
pGEMTeasy | Cloning vector used in E. coli; 3.0 kb; Ampr | Promega |
pGadB-A | pGEMTeasy containing 1 kb of the DNA sequence upstream of gadB; 4.0 kb; Ampr | This study |
pGadB-B | pGEMTeasy containing 0.9 kb of the DNA sequence downstream of gadB; 3.9 kb; Ampr | This study |
pGadB-AB | pGEMTeasy containing the upstream and downstream sequences of gadB; 4.9 kb; Ampr | This study |
pJRS233 | Shuttle vector used in the hosts E. coli and L. reuteri 100-23; 6.0 kb; Ermr | [29] |
pKO-gadB-AB | pJRS233 containing 1.9 kb of the flanking sequences of gadB; 7.9 kb; Ermr | This study |