Table 1 Primer sequences used for qRT-PCR.
Target gene | Forward primer | Reverse primer | Amplicon length (bp) |
|---|---|---|---|
adhE | CTGACCCAACTTTGAGCGAAGC | AGAGCAGAACCACCACCAAGAC | 94 |
busAB | TTCCAGCGGTTGCATTCTTTGG | TTCTGACTGTTGGTGGGAGTGC | 84 |
clpE | GCAGCAAGCACTCAAACTCCAC | ACGACGAGCTGACTCTGTGATG | 132 |
glpF1 | AATGGGGTTGTCGCAGCAAATG | AGAAGCCAACTACCCAGACAGC | 118 |
ilvD | CAACACAACCAGCGACTCAAGC | ATGCTGACGATTCCGACCTGAG | 88 |
ldh | ATGGTGTCGCTGTAGCTCTTG | GCAGTCGCTTACGCCATATTG | 110 |
optC | TTTGCATGGACCGGGATGGC | TGAGGCCAAGACGAAGTCACG | 78 |
ptnAB | CTGCTGTTCAAGGTGCGATTCC | ACGATGATGCGGTTTGCTCTTG | 145 |
rplM | TGGTACGTCGTCGATGCAACTG | TTTTCCACGAAGTACGCTTGCG | 75 |
rpoB | TCCTGATGCAGAATGCCGTGTC | TCAGCAAGACCAAGCCAGTAGC | 75 |