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Table 1 Strains used in this study, their origin and effects of the different growth conditions on the on pilus gene/protein expression.

From: Genetic analysis and morphological identification of pilus-like structures in members of the genus Bifidobacterium

Organism

Origin

Pilus gene cluster

Carbon sources

   

Glucose*

lactose

FOS

mucin

Bovine milk

N-acetylglucosamine

   

AFM

qRT-PCR

AFM

qRT-PCR

AFM

qRT-PCR

AFM

qRT-PCR

AFM

qRT-PCR

AFM

qRT-PCR

B. bifidum PRL2010

Feces of breast-fed infant

282-284

 

nd

 

nd

 

nd

 

nd

 

yes

 

nd

  

1707-1709

+

nd

+

nd

+

yes

-

nd

-

nd

-

nd

  

1820-1822

 

nd

 

nd

 

nd

 

nd

 

yes

 

nd

B. longum subsp. infantis ATCC15697

Intestine of infant

-

nd

-

+

 

nd

 

-

 

-

 

-

 

B. dentium Bd1

Dental caries

142-144

 

nd

 

nd

 

nd

 

nd

 

nd

 

nd

  

197-200

 

nd

 

nd

 

nd

 

nd

 

nd

 

nd

  

276-278

 

-

 

-

 

-

 

-

 

-

 

-

  

534-536

+

-

+

-

nd

-

-

-

-

-

-

-

  

1874-1876

 

-

 

-

 

-

 

-

 

-

 

-

  

2000-2002

 

nd

 

yes

 

nd

 

nd

 

nd

 

nd

  

2188-2191

 

-

 

-

 

-

 

-

 

-

 

-

B. animalis subsp. lactis DSM10140

Yogurt

1488-1486

+

nd

+

+

+

+

-

nd

-

nd

-

nd

B. adolescentis ATCC15703

Intestine of adult

1467-1470

nd

nd

+

nd

+

+

-

nd

-

+

-

+

  1. *Glucose was used as reference condition for normalizing the qRT-PCR experiments; nd, not identified; +, identified ; -, not performed or present in the genome; ATCC, American Type Culture Collection; DSM, Deutsche Sammlung von Mikroorganismen