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Table 2 Specific activity and K m determination of the secondary alcohol dehydrogenases.

From: Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli

Gene

Methylglyoxal

Hydroxyacetone

 

Specific Activity

(U/mg)

K m

(mM)

Specific Activity

(U/mg)

K m

(mM)

gldA

2.45 ± 0.00

68.24 ± 0.05

0.91 ± 0.00

10.47 ± 0.55

budC

3.71 ± 0.06

0.78 ± 0.03

4.97 ± 0.00

1.83 ± 0.63

  1. The decrease in absorbance of NADH at 340 nm was recorded and used for calculations using the substrates methylglyoxal and hydroxyacetone. Substrate concentration was varied from 20 mM - 120 mM. 1 unit (U) was defined as the amount (μmoles) of product formed per unit time (min).
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Microbial Cell Factories

ISSN: 1475-2859

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