Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli

Table 1 Methylglyoxal synthase assay results.

mgsA source	Specific Activity (U/mg)	K_m(mM)	Specific Activity/K_m(U/mg/mM)
C. acetobutylicum	0.054 ± 0.004	0.776 ± 0.005	0.069
*B. subtilis*	0.056 ± 0.003	0.473 ± 0.07	0.118
C. difficile	0.059 ± 0.0.003	1.439 ± 0.06	0.041
E. coli	0.124 ± 0.006	1.418 ± 0.12	0.087
T. thermophilus	0.016 ± 0.004	2.118 ± 0.07	0.007
K. pneumoniae	0.016 ± 0.009	2.820 ± 0.3	0.005
P. fluorescens	0.013 ± 0.008	1.560 ± 0.02	0.008
R. eutropha	0.005 ± 0.000	0.700 ± 0.03	0.007

Substrate dihydroxyacetone phosphate concentration was varied from 0.15 mM to 1.5 mM for all reactions. 1 unit (U) was defined as the amount (μmoles) of methylglyoxal formed per unit time (min).

ISSN: 1475-2859