Table 2 pH-Stability of wild-type L-AI and its T268K mutant (values within brackets) at different temperatures without addition of metal ions.

Half-lives ( t _1/2 , h)

3.0

27 ± 0.2 (39 ± 0.1)

28 ± 0.1 (38 ± 0.3)

27 ± 0.4 (38 ± 0.7)

27 ± 0.1 (38 ± 0.4)

3.5

32 ± 0.3 (39 ± 0.7)

33 ± 0.5 (39 ± 0.2)

32 ± 0.1 (39 ± 0.1)

31 ± 0.2 (40 ± 0.5)

4.0

35 ± 0.4 (40 ± 0.3)

34 ± 0.4 (41 ± 0.7)

35 ± 0.4 (41 ± 0.4)

36 ± 0.3 (41 ± 0.8)

4.5

39 ± 0.6 (42 ± 0.5)

39 ± 0.4 (43 ± 0.4)

38 ± 0.8 (43 ± 0.2)

39 ± 0.6 (42 ± 0.3)

5.0

41 ± 0.2 (42 ± 0.4)

42 ± 0.3 (43 ± 0.2)

40 ± 0.5 (44 ± 0.8)

41 ± 0.8 (42 ± 0.9)

5.5

43 ± 0.7 (43 ± 0.8)

44 ± 0.2 (44 ± 0.6)

44 ± 0.6 (44 ± 0.2)

43 ± 0.4 (43 ± 0.2)

6.0

43 ± 0.5 (43 ± 0.2)

44 ± 0.6 (44 ± 0.8)

44 ± 0.7 (44 ± 0.5)

43 ± 0.7 (43 ± 0.4)

6.5

43 ± 0.2 (44 ± 0.6)

44 ± 0.1 (44 ± 0.5)

44 ± 0.5 (44 ± 0.6)

43 ± 0.4 (44 ± 0.5)

7.0

44 ± 0.8 (44 ± 0.4)

44 ± 0.8 (44 ± 0.2)

44 ± 0.2 (44 ± 0.3)

43 ± 0.1 (44 ± 0.1)

7.5

43 ± 0.2 (43 ± 0.6)

43 ± 0.3 (43 ± 0.4)

43 ± 0.1 (43 ± 0.3)

43 ± 0.5 (43 ± 0.4)

8.0

41 ± 0.5 (41 ± 0.6)

41 ± 0.6 (42 ± 0.3)

42 ± 0.2 (42 ± 0.1)

42 ± 0.7 (42 ± 0.6)

8.5

40 ± 0.6 (40 ± 0.5)

40 ± 0.7 (40 ± 0.8)

40 ± 0.5 (40 ± 0.7)

40 ± 0.7 (40 ± 0.9)