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Table 1 Biochemical properties of previously reported L-AIs.

From: The acid-tolerant L-arabinose isomerase from the mesophilic Shewanella sp. ANA-3 is highly active at low temperatures

Organism

Topt (°C)

pH

Metal ion

Requirement (mM)

K m (mM)

V max (mmole/s/mg)

References

Shewanella sp. ANA-3

15-35

5.5-6.5

Mn2+ (0.6)

33.7*

164*

This study

Acidothermus cellulolytics

75

7.5

Mn2+ (1.0), Co2+ (0.5)

NR

NR

[35]

Alicyclobacter acidocaldarius

50

6.0

Mn2+ (1.0), Co2+, (1.0) Mg2+ (1.0)

48

35.5

[15]

Anoxybacillus flavithermus

95

9.5-10.5

Ni2+ (1.0)

78.5

94.3

[25]

Bacillus stearothermophilus US100

80

7.5-8.0

Mn2+ (1.0), Co2+ (0.2)

28.57

185

[13]

Bacillus halodurans

50

7.5-8.0

Mn2+ (1.0)

36

33.1

[27]

Bacillus licheniformis

50

7.5

Mn2+ (1.0), Co2+ (1.0)

369

NR

[14]

Bacillus subtilis

32

7.5

Mn2+ (1.0)

120

NR

[36]

Escherichia coli

30

8.0

Mn2+(0.5)

60

NR

[37]

Geobacillus stearothermophilus T6

70

7.0-7.5

Mn2+ (1.0)

63

36.5

[1]

Geobacillus thermodenitrificans

60

7.5

Mn2+ (5.0), Co2+ (3.0)

142

86

[12]

Lactobacillus fermentum

65

6.5

Mn2+ (1.0), Co2+ (2.0)

29.9

24.3

[38]

Lactobacillus plantarum NC8

60

7.5

Mn2+ (1.0), Co2+ (0.5)

43.42

40

[21]

Lactobacillus sakei 23 K

30-40

5.0-7.0

Mg2+ (0.8), Mn2+ (0.8)

31.6

264

[24]

Thermotoga maritima

90

7.0

Mn2+(5.0), Co2+(1.0)

63

41.3

[26]

Thermotoga neapolitana

85

7.0-7.5

Mn2+ (1.0), Co2+ (1.0)

60

119

[10]

Thermus sp. IM6501

60

8.5

Mn2+ (5.0)

NR

NR

[18]

Bacillus stearothermophilus IAM1101

65

6.0

MnP2+P (1.0)

NR

NR

[39]

  1. NR: Not Reported
  2. Top: Optimal temperature
  3. *: Substrate is L-arabinose