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Figure 4 | Microbial Cell Factories

Figure 4

From: A novel esterase gene cloned from a metagenomic library from neritic sediments of the South China Sea

Figure 4

Purification of recombinant Est_p1. (A) Proteins recovered during various purification steps as described in the text were separated by SDS-10% polyacrylamide gel electrophoresis, and stained with Coomassie Brilliant Blue R-250. Lane M, molecular weight standards; Lane CL, induced cell lysate; Lane FT, flow-through; Lane EL, 50 mM imidazole elution. (B) Silver staining of purified Est_p1. Protein size markers are indicated (kilo Daltons, kDa) at left. Recombinant Est_p1 proteins are indicated by arrow at right.

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Microbial Cell Factories

ISSN: 1475-2859

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