Table 2 PCR primers used in this study
Primer name | Sequence (5'-3') | Orientation | Use |
|---|---|---|---|
LChi (F) | GAGAATCGCAACTGACTT | Forward | RT-PCR |
LChi (R) | GCAGGTGAATTCAGACGG | Reverse | RT-PCR |
3-RC | AGGAAACCAGATCGGGATGT ACTGGCC | Forward | RACE amplification |
5-RC | GTCGTCGCGCTCCAGGAGCCATAAAT | Reverse | RACE amplification |
CHI-For | ATGAAGATCCCTCTCCTCTCCTCC | Forward | RT-PCR |
CHI-Rev | CTACGCCTTGAACGTCCCCGCCAGT | Reverse | RT-PCR |
CHI-Not I | TTTGCGGCCGC CTCACCCCTACCACTCCCTC | Forward | PCR |
CHI-Xba I | TTTTCTAGA AACGCCTTGAACGTCC CCGCCAGT | Reverse | PCR |