Figure 5From: Molecular cloning, expression and biochemical characterisation of a cold-adapted novel recombinant chitinase from Glaciozyma antarctica PI12Purification of recombinant chitinase (CHI II). Approximately 20 μl of sample was loaded into each well. M: protein molecular mass marker; Lane 1: crude concentrated enzyme; Lane 2 & 3: unbound fraction; Lane 3 & 4: elution fraction.Back to article page