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Figure 4 | Microbial Cell Factories

Figure 4

From: Molecular cloning, expression and biochemical characterisation of a cold-adapted novel recombinant chitinase from Glaciozyma antarctica PI12

Figure 4

SDS-PAGE, western blot and glycosylation analysis of recombinant CHI II protein in P. pastoris GS115. (a) SDS-PAGE of transformant, M: protein molecular mass marker; Lanes 1-5: transformants induced by methanol for 1 to 5 days; Lane 6: control, GS115 transformed with empty pPICZαA and induced by methanol for 5 days. (b) Western blot of transformant, Lanes 1-5: transformants induced by methanol for 1 to 5 days; Lane 6: control, GS115 transformed with empty pPICZαA and induced by methanol for 5 days. (c) Glycosylation analysis of transformant, Lanes 1-2: transformants induced by methanol for 4 to 5 days; Lane 3: Horseradish Peroxidase Positive Control; Lane 4: Soybean Trypsin Inhibitor Negative Control. A 50 kDa protein was positively stained and indicated by an arrow.

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