Table 4 List of primers used for quantification of gene dosage and transcriptional levels by qRT-PCR
Target gene | Primer name | Primer sequence |
|---|---|---|
ERO1 | 5'ERO1_qPCR_vie | 5' GTTGGAAAAGCCGCATATAAACAAAACA |
3'ERO1_qPCR_vie | 5' CAGCTTGGGCAAAGTCCTGTAAGAGTTC | |
WSC4 | 5'WSC4_qPCR | 5' CAGCACCATCCATATCAACC |
3'WSC4_qPCR | 5' GTTTGCGGATCTTGAGCTAC | |
ACT1 | 5'ACT1_qPCR_vie | 5' CCTGAGGCTTTGTTCCACCCATCT |
3'ACT1_qPCR_vie | 5' GGAACATAGTAGTACCACCGGACATAACGA | |
2F5_HC | 5'2F5_HC_qPCR | 5' CTCTCACGCTGACCTGTTCC |
3'2F5_HC_qPCR | 5' GATTGCAAGCCACTCTAGGG | |
2F5_LC | 5'2F5_LC_qPCR | 5' CTTCCCGCCATCTGATGAGC |
3'2F5_LC_qPCR | 5' GAGGGCGTTATCCACCTTCC |