Table 3 List of primers used for amplification of genes for co-expression in P.pastoris Fab 2F5
Target gene | Primer name | Primer sequence |
|---|---|---|
ERO1 | ERO1_fwd | 5' AACTGCCTGCAGG ACC_ATGAGGATAGTAAGGAGCGTAGCTAT |
ERO1_rev | 5' AATCGGGCCGAGGCGGCC _TTACAAGTCTACTCTATATGTGGTATCT | |
NCE103 | NCE103_fwd | 5' AAGCGCCTGCAGG ACC_ATGGGTGGTTTATCATTTGA |
NCE103_rev | 5' AATCGGGCCGAGGCGGCC _TTAATGTCCACCGGCTTCAGTATCA | |
AQR1 | QDR2_fwd | 5' AACTGCCTGCAGG ACC_ATGACAAATGAAAAATTGGATTTG |
QDR2_rev | 5' AATCGGGCCGAGGCGGCC _CTACAGTTTGTATTTTGTTCCCCTCCTA | |
SLY41 | SLY41_fwd | 5' AACTGCCTGCAGG ACC_ATGATCATTACGCAGAATCT |
SLY41_rev | 5' AATCGGGCCGAGGCGGCC _CTAGTTTTTGACTGCACCCCATTT | |
TDH3 | TDH3_fwd | 5' AAGCGCCTGCAGG ACC_ATGGCTATCACTGTCGGTAT |
TDH3_rev | 5' AATCGGGCCGAGGCGGCC _TTAAGCCTTAGCAACGTGTT | |
TEF4 | TEF4_fwd | 5' AACTGCCTGCAGG ACC_ATGTCGCAAGGAACAATTTAC |
TEF4_rev | 5' AATCGGGCCGAGGCGGCC _TTAATTACTCTTGGGTGGAACAT | |
TSA1 | TSA1_fwd | 5' AACTGCCTGCAGG ACC_ATGTTTGGACTAAATCACGAGATA |
TSA1_rev | 5' AATCGGGCCGAGGCGGCC _CTATTTGGACTTGGAAAAGAA | |
WSC4 | WSC4_fwd | 5' AACTGCCTGCAGG ACC_ATGTTGTTGAAGTTGATTTGGGTATTT |
WSC4_rev | 5' AATCGGGCCGAGGCGGCC _TTAGGCATTATTTCCTGGGGTCTCT |