Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

Table 2 Composition of major sterols in both untreated and fluconazole-treated P. pastori s cells

Sterol	μg Sterol/mg DCW
	Control culture	Fluconazole-treated culture
Unknown 1	0	0.5
Unknown 2	0	0.47
Squalen	0	0
Zymosterol	0.17	0
Ergosterol	9.81	6.77
Fecosterol	0.21	0
Episterol	0.23	0
Total sterols	10.42	7.74

Amounts of sterol (μg_sterol mg_DWC^-1) were determined from P. pastoris X-33 pGAPZαA Fab2F5 cells treated with 0.6 μg ml^-1 fluconazole and compared with untreatred cells. Unknown 1 and 2 refer to non-identified compounds detected in GC analyses of the sterol fraction of fluconazole-treated P. pastoris cells (see Additional file 1).

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