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Table 1 List of target genes for co-expression in P.pastoris Fab 2F5

From: Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

Target gene ORF Description fold change adj p-value
ERO1 PIPA00063 Thiol oxidase required for oxidative protein folding in the endoplasmic reticulum 6.49 9.15E-07
NCE103 * PIPA03864 Carbonic anhydrase, involved in non-classical protein export pathway 3.36 4.08E-08
AQR1 * PIPA04502 Plasma membrane multidrug transporter of the major facilitator superfamily 17.03 2.25E-09
SLY41 PIPA02527 Protein involved in ER-to-Golgi transport 1.85 3.22E-04
TDH3 PIPA02510 Glyceraldehyde-3-phosphate dehydrogenase, involved in glycolysis and gluconeogenesis 4.38 5.44E-06
TEF4 PIPA00834, PIPA10574 Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA to ribosomes 36.25 6.15E-10
TSA1 PIPA04168 Thioredoxin peroxidase, self-associates to form a high-molecular weight chaperone complex under oxidative stress 5.78 2.83E-06
WSC4 PIPA00592 ER membrane protein involved in translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane 2.57 8.82E-05
  1. Target genes (systematic names and P. pastoris specific PIPA codes) for co-expression are listed together with their biological function. The fold change ratios and adjusted p-values derive from the pairwise comparison of hypoxic and normoxic conditions. Target genes highlighted with (*) are also significantly (p-value < 0.05) upregulated in the Fab expressing strain compared to the control strain in hypoxic conditions.