Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

Table 1 List of target genes for co-expression in P.pastoris Fab 2F5

Target gene	ORF	Description	fold change	adj p-value
ERO1	PIPA00063	Thiol oxidase required for oxidative protein folding in the endoplasmic reticulum	6.49	9.15E-07
NCE103 *	PIPA03864	Carbonic anhydrase, involved in non-classical protein export pathway	3.36	4.08E-08
AQR1 *	PIPA04502	Plasma membrane multidrug transporter of the major facilitator superfamily	17.03	2.25E-09
SLY41	PIPA02527	Protein involved in ER-to-Golgi transport	1.85	3.22E-04
TDH3	PIPA02510	Glyceraldehyde-3-phosphate dehydrogenase, involved in glycolysis and gluconeogenesis	4.38	5.44E-06
TEF4	PIPA00834, PIPA10574	Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA to ribosomes	36.25	6.15E-10
TSA1	PIPA04168	Thioredoxin peroxidase, self-associates to form a high-molecular weight chaperone complex under oxidative stress	5.78	2.83E-06
WSC4	PIPA00592	ER membrane protein involved in translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane	2.57	8.82E-05

Target genes (systematic names and P. pastoris specific PIPA codes) for co-expression are listed together with their biological function. The fold change ratios and adjusted p-values derive from the pairwise comparison of hypoxic and normoxic conditions. Target genes highlighted with (*) are also significantly (p-value < 0.05) upregulated in the Fab expressing strain compared to the control strain in hypoxic conditions.

ISSN: 1475-2859