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Figure 4 | Microbial Cell Factories

Figure 4

From: Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

Figure 4

Fluconazole screening. A: Determination of the fluconazole working concentrations. Growth curves of the recombinant P. pastoris strain X-33 pGAPZαA Fab2F5 grown in BMD medium and in the presence of 0 - 2 μg ml-1 fluconazole. B: Fluconazole screening in shake flask cultures. Fab productivities in different concentrations of fluconazole (0.2 - 1.0 μg ml-1) were normalized to the values obtained from non-treated cells. The relative Fab productivities for each concentration are demonstrated, error bars indicate the standard error of the means.

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