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Figure 1 | Microbial Cell Factories

Figure 1

From: Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

Figure 1

24-well plate screening of co-expressing strains. Eight individual clones of each target gene and of a control strain containing the empty vector were used for a small scale screening in 24-well plates. All cultures started with the same initial OD600 of 0.1 in BMD medium containing 2% (w/v) glucose. After 24 hours, Fab titers and wet cell weight were calculated to determine the product yield (mgFab gWCW-1) at a given cultivation point (24 h). The yield ratios relative to the control strain (value = 1) are illustrated. Error bars indicate the standard error of the means.

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