Figure 1

24-well plate screening of co-expressing strains. Eight individual clones of each target gene and of a control strain containing the empty vector were used for a small scale screening in 24-well plates. All cultures started with the same initial OD₆₀₀ of 0.1 in BMD medium containing 2% (w/v) glucose. After 24 hours, Fab titers and wet cell weight were calculated to determine the product yield (mg_Fab g_WCW^-1) at a given cultivation point (24 h). The yield ratios relative to the control strain (value = 1) are illustrated. Error bars indicate the standard error of the means.