From: Modifications of the metabolic pathways of lipid and triacylglycerol production in microalgae
Modification | Organism | Result | Reference |
---|---|---|---|
Expression of a cytosolic variant of endogenous ACCase | Brassica napus | 5% increase in seed oil content | [40] |
Expression of KASIII from Spinacia oleracea | Brassica napus | Increased palmitic acid proportion, decreased total fatty acids 5-10% | [41] |
Saccharomyces cerevisiae G3p dehydrogenase (gpd1)expression | Brassica napus | 40% increase in seed oil content | [43] |
Carthamus tinctorius G3p acyltransferase (GPAT) expression | Arabidopsis thaliana | 10-21% increase in seed oil content | [45] |
Saccharomyces cerevisiae sn-2 acyltransferase (SLC1-1) expression | Brassica napus | 53-121% increase in erucic acid content | [47] |
Arabidopsis thaliana diacylglycerol acyltransferase (DGAT1) expression | Brassica napus | Increases in oil content and seed weight | [48] |
Down regulation of FAD2 desaturase and FatB hydrolase | Glycine max | 85% increase in oleic acid levels | |
Expression of Coriandrum sativum Δ4palmitoyl ACP desaturase | Nicotiana tabacum | < 10% of total fatty acid became palmitoleic acid | [56] |
Expression of Thunbergia alata Δ6 ACP desaturase | Arabidopsis thaliana | < 10% of total fatty acid became palmitoleic acid | [57] |
Expression of Umbellularia californica lauryl-ACP thioesterase | Arabidopsis thaliana | 24% of total fatty acid converted to laurate | [66] |
Expression of Umbellularia californica lauryl-ACP thioesterase | Brassica napus | 58% of total fatty acid converted to laurate | [67] |
Expression of Cuphea hookeriana FatB1 thioesterase | Brassica napus | Fatty acid content changed to 11% caprylate and 27% caprate | [68] |
Co-expression of Cuphea hookeriana FatB1 thioesterase and KAS (ketoacyl ACP synthase) | Brassica napus | 30-40% increase in short chain fatty acid content over FatB1 expression only | [69] |
Co-expression of Cuphea hookeriana FatB1 thioesterase and LPAAT from Cocos nucifera | Brassica napus | 67% of total fatty acid content converted to laurate | [70] |