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Table 1 Comparison of kinetic parameters of huIFN α2b production under different induction strategies

From: Development of a cultivation process for the enhancement of human interferon alpha 2b production in the oleaginous yeast, Yarrowia lipolytica

Parameter

Strategy

 

1

2

3

4

St (g L-1)

20

10

20

20

Si (g L-1)

20

10

5

a

Induction period (h)

55

54

33

21

Sc (g L-1)

11.14

6.3

13.8

17.3

X (g L-1)

84.7

92.3

83.7

65

YX/S (g g-1)

0.96

3.06

0.77

0

huIFNα2b (mg L-1)b

185

207

280

355

V (mg L-1 h-1)

3.36

3.83

8.5

16.9

P (mg g-1 h-1)

0.04

0.041

0.1

0.26

  1. a Oleic acid was add by a continuous feeding, at a rate of 1.25 g h-1
  2. b Mean level of huIFNα2b
  3. St: total oleic acid concentration, Si: initial oleic acid concentration, Sc: substrate consumed. X: mean biomass level during induction, V: mean volumetric production of huIFN α2b, P: mean specific production of huIFN α2b
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Microbial Cell Factories

ISSN: 1475-2859

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