Table 1 Strains and plasmids used in this study.
Strain | Plasmid | Phenotype | Source |
|---|---|---|---|
CE | pCEL15 | Secretes a small peptide (negative control) | Tsai et al, 2010 |
AT | pAt | Secretes the endoglucanase At (CelA from C. thermocellum with its native dockerin) | Tsai et al, 2010 |
CB | pCBH2c | Secretes the cellobiohydrolase CBHc (CBHII from T. reesei fused with a dockerin from C. cellulolyticum) | Tsai et al, 2010 |
BF | pBGLf | Secretes the β-glucosidase Bglf (Bg1I from T.aurantiacus fused with a dockerin from R. flavefaciens) | Tsai et al, 2010 |
SC | pAgα-scaf3 | Display of Scaf-ctf by an Agα1 anchor in a centromeric plasmid | This study |