Figure 3

Cell growth and ethanol production by the cell consortia. (A) Cell growth and (B) PASC hydrolysis (dotted line) and ethanol production (solid line) by the different yeast consortia, i.e., consortium C2 without secreting enzymes (♦), consortium C3 only secreting enzymes (●) and consortium C1 forming the cellulosome structure (■). (C) Surface display of the mini-cellulosome was probed with either anti-C-myc sera for the displayed scaffoldin or anti-C-His6 sera for the three cellulases docked on the scaffoldin and fluorescently stained with a goat anti-mouse IgG conjugated with Alexa Fluor 488. Whole cell fluorescence was determined using a fluorescent microplate reader. Data shown are the mean values (± standard deviation) obtained from 3 independent experiments.