Table 2 Primers used in this study
Primers | Sequences (5'-3') | Source |
|---|---|---|
CbsA2F | GTACCAAGCCAAAGCAAGAC | [30] |
CbsA2R | GTTTGAAGCCTTTACGTAAGTC | [29] |
SF | CAAGTTCATCAACGCTGACAACATCG | This study |
SR | GTAGTATGCATTGTGCATAATAGTCT | This study |
SlpF | ACATTTTTTATATTTCAAGGAGGA | This study |
SlpR | CGTTTTCCTTATATACTACTTAGACT | This study |
8F | AGAGTTTGATCCTGGCTCAG | [35] |
1541R | AAGGAGGTGATCCAGCCGCA | [35] |
LcsB-HindIII | GCTAAGCTT AATTGGTTCGTTGCAC | This study |
LcsB-XhoI | GTGCTCGAG GTTTGAAGCCTTTACGT | This study |
GFP-NisI | GAGATGCAT ATATGAGCAAAGGAGAAG | This study |
Gal-NsiI | CGCATGCAT TGATTAGCAGCAAATTACC | [38] |
LcsB-EcoRI | GCGGAATTC TTAAAAGTTTGAAGCCTTTACGT | This study |
GFP-EcoRI | CGCGAATTC TTAGTAGAGCTCATC | This study |
Cm | ATGGACTTCATTTACTGGGTTTA | This study |