Table 1 Strains, plasmids used in this study.
Strain | Decription | Source |
|---|---|---|
E. coli DH5α | supE44ΔlacU169φ80lacZΔM15hsdR17recA1endA1gyrA96thi-1relA1 | Novagen |
E. coli Origami B (DE3) | F-ompT hsdSB (rB- mB-) gal dcm lacY1 ahpC gor522::Tn10 (TcR) trxB::Kan (DE3) | Novagen |
Lb. crispatus K2-4-3 | Isolated from chicken intestine | Our lab |
Lb. johnsonii K23 | Isolated from chicken intestine | Our lab |
Lb. salivarius | Isolated from chicken intestine | Our lab |
L. lactis NZ9000 | MG1363 pepN::nisRK. Most commonly used host of the NICE system | [27] |
Lb. brevis K31 | Wild type | Our lab |
Lb. casei BL23 | Kindly gifted by Hazebrouck | [36] |
S. thermophilus K11 | Wild type | Our lab |
Lb. helveticus K17 | Wild type | Our lab |
Lb. delbrueckii subsp. bulgaricus ATCC 11842 | Kindly gifted by M. van de Guchte | [37] |
Plasmid | Â | Â |
pSec:Leiss:Nuc | pGK:Cmr; mature Nuc fused at 5' end with sequence of propeptide LEISSTCDA under the control of PnisA | [25] |
pLeiss:GFP | pSec:Leiss:Nuc(NsiI/EcoRI::gfp) | This study |
pLeiss:GFP:LcsB | pSec:Leiss:Nuc(NsiI/EcoRI::gfp-lcsB) | This study |
pET-gfp | Apr,pET-22b(+)(NdeI/HindIII::gfp-Taghis6) | [15] |
pET-gfp-lcsB | Apr,pET-22b(+)(NdeI/XhoI::gfp-lcsB-Taghis6) | This study |
pET-gal | Apr,pET-22b(+)(NdeI/HindIII::gal-Taghis6) | [15] |
pET-gal-lcsB | Apr,pET-22b(+)(NdeI/XhoI::gal-lcsB-Taghis6) | This study |