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Table 2 Batch experiments with methanol pulses to determine strain specific parameters of different P. pastoris strains.

From: A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains

 

KM71H

KM71H HRPa

KM71H PDI HRP

CBS7435 HRP

SMD1168H GalOX

Δtimeadapt Batch [h]

3.5

7

7

7.5

14

max. qCO2' [h]

2.1

2.3

5.7

2.5

2.7

qs adapt

[mmol·g-1·h-1]

0.96 ± 0.10

0.80 ± 0.08

0.56 ± 0.06

0.77 ± 0.08

0.48 ± 0.05

qs maxb

[mmol·g-1·h-1]

1.94 ± 0.19

2.00 ± 0.20

1.08 ± 0.10

1.54 ± 0.15

2.62 ± 0.26

qp maxb

[U·g-1·h-1]

-

2.5 ± 0.25

6.3 ± 0.63

4.25 ± 0.43

200.8 ± 20.1

  1. a data taken from [18]
  2. b representing the maximum value determined out of all pulses