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Table 2 Batch experiments with methanol pulses to determine strain specific parameters of different P. pastoris strains.

From: A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains

  KM71H KM71H HRPa KM71H PDI HRP CBS7435 HRP SMD1168H GalOX
Δtimeadapt Batch [h] 3.5 7 7 7.5 14
max. qCO2' [h] 2.1 2.3 5.7 2.5 2.7
qs adapt
[mmol·g-1·h-1]
0.96 ± 0.10 0.80 ± 0.08 0.56 ± 0.06 0.77 ± 0.08 0.48 ± 0.05
qs maxb
[mmol·g-1·h-1]
1.94 ± 0.19 2.00 ± 0.20 1.08 ± 0.10 1.54 ± 0.15 2.62 ± 0.26
qp maxb
[U·g-1·h-1]
- 2.5 ± 0.25 6.3 ± 0.63 4.25 ± 0.43 200.8 ± 20.1
  1. a data taken from [18]
  2. b representing the maximum value determined out of all pulses