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Figure 3 | Microbial Cell Factories

Figure 3

From: Characterization of DNA polymerase β from Danio rerio by overexpression in E. coli using the in vivo/in vitro compatible pIVEX plasmid

Figure 3

Expression of Danio pol β in vivo and in vitro. (A) SDS-PAGE analysis of the proteins from E. coli KRX at each step of the expression and purification procedures. Lane M, molecular weight marker, Lane 1, uninduced cell extract (0 h); Lane 2, uninduced cell extract (12 h): Lane 3, induced cell extract (12 h); Lane 4, elute from Ni2+-chilete column chromatography. Proteins were stained with Coomassie brilliant blue (CBB). (B) Western blotting analysis of recombinant Danio pol β expressed in vivo (lane 1) and. in vitro (lane 2)

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