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Table 3 Activity of P mtlA (pKAM12) and P mtlR (pKAM18) in B. subtilis 3NA and mutants thereof

From: Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors

  B. subtilis
Treatment (0.2%) 3NA pKAM12 KM12 pKAM12 KM15 pKAM12 KM37 pKAM12 KM163 pKAM12 3NA pKAM18 KM12 pKAM18 KM15 pKAM18 KM37 pKAM18
Mannitol 13178 ± 1563 13862 ± 1342 92 ± 49 10801 ± 3290 13722 ± 1231 95 ± 25 243 ± 34 16 ± 1 156 ± 39
Mannitol + Glucose 5920 ± 894 10289 ± 1131 61 ± 46 1921 ± 967 11795 ± 729 28 ± 18 143 ± 14 5 ± 1 53 ± 21
Glucose 482 ± 86 10493 ± 1569 57 ± 42 1021 ± 379 6208 ± 583 14 ± 4 135 ± 39 5 ± 1 24 ± 4
Mannitol + Xylose 13693 ± 1343 14084 ± 2081 81 ± 45 11762 ± 3814 12499 ± 2112 99 ± 30 214 ± 33 15 ± 2 148 ± 49
Xylose 555 ± 120 14046 ± 2391 84 ± 52 2834 ± 943 1879 ± 76 25 ± 3 217 ± 41 15 ± 1 46 ± 7
Glucitol 8009 ± 947 12519 ± 1012 81 ± 53 11841 ± 3728 12425 ± 3297 51 ± 26 192 ± 43 10 ± 1 143 ± 42
Glucitol + Glucose 4559 ± 555 10743 ± 663 65 ± 51 1492 ± 611 11555 ± 2364 26 ± 22 142 ± 36 6 ± 1 44 ± 13
Uninduced 594 ± 83 15056 ± 2420 95 ± 59 2838 ± 933 2389 ± 645 23 ± 4 265 ± 71 16 ± 1 47 ± 10
  1. The strains were induced by 0.2% (w/v) of sugars, or no inducer was added (control). β-galactosidase activity was measured 1 h after addition of inducer. Activity was calculated as Miller units. Mutations: KM12 (ΔmtlAF); KM13 (ΔmtlAFD); KM15 (ΔmtlR); KM37 (mtlD::ermC); KM163 (mtlR-H342D).