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Table 3 Activity of P mtlA (pKAM12) and P mtlR (pKAM18) in B. subtilis 3NA and mutants thereof

From: Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors

 

B. subtilis

Treatment (0.2%)

3NA pKAM12

KM12 pKAM12

KM15 pKAM12

KM37 pKAM12

KM163 pKAM12

3NA pKAM18

KM12 pKAM18

KM15 pKAM18

KM37 pKAM18

Mannitol

13178 ± 1563

13862 ± 1342

92 ± 49

10801 ± 3290

13722 ± 1231

95 ± 25

243 ± 34

16 ± 1

156 ± 39

Mannitol + Glucose

5920 ± 894

10289 ± 1131

61 ± 46

1921 ± 967

11795 ± 729

28 ± 18

143 ± 14

5 ± 1

53 ± 21

Glucose

482 ± 86

10493 ± 1569

57 ± 42

1021 ± 379

6208 ± 583

14 ± 4

135 ± 39

5 ± 1

24 ± 4

Mannitol + Xylose

13693 ± 1343

14084 ± 2081

81 ± 45

11762 ± 3814

12499 ± 2112

99 ± 30

214 ± 33

15 ± 2

148 ± 49

Xylose

555 ± 120

14046 ± 2391

84 ± 52

2834 ± 943

1879 ± 76

25 ± 3

217 ± 41

15 ± 1

46 ± 7

Glucitol

8009 ± 947

12519 ± 1012

81 ± 53

11841 ± 3728

12425 ± 3297

51 ± 26

192 ± 43

10 ± 1

143 ± 42

Glucitol + Glucose

4559 ± 555

10743 ± 663

65 ± 51

1492 ± 611

11555 ± 2364

26 ± 22

142 ± 36

6 ± 1

44 ± 13

Uninduced

594 ± 83

15056 ± 2420

95 ± 59

2838 ± 933

2389 ± 645

23 ± 4

265 ± 71

16 ± 1

47 ± 10

  1. The strains were induced by 0.2% (w/v) of sugars, or no inducer was added (control). β-galactosidase activity was measured 1 h after addition of inducer. Activity was calculated as Miller units. Mutations: KM12 (ΔmtlAF); KM13 (ΔmtlAFD); KM15 (ΔmtlR); KM37 (mtlD::ermC); KM163 (mtlR-H342D).
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Microbial Cell Factories

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