Table 2 Oligonucleotides used in this study
Name | Sequence (5'→3') | Purpose |
|---|---|---|
Expression vectors | Â | |
s5526 | AAAAAAGCTAGCGGCTCCTGAAACCAGGAG | Amplification of P mtlA |
s5527 | AAAAACTTAAGATATAAACCCTCCCTGTTTTG | Amplification of P mtlA |
s5799 | AAGCTAGCTACGATATTCCATAAAAAGC | Amplification of P mtlR |
s6209 | AAAAAGCTAGCTTTTTATTTTTAAAAAATTGTCACAGTCA | Shortening upstream of P mtlA |
s6210 | AAAAAAGCTAGCTAAAAAATTGTCACAGTCATGTGC | Shortening upstream of P mtlA |
s6213 | AAAAAACTTAAGTAAGATACAAAAATATGTTCAGAGA | Shortening downstream of P mtlA |
s6392 | AAAAAACTTAAGAGCCAATCTTGATGTGCGG | Amplification of P mtlR |
s6688 | AAAAAAGCTAGCTTTTTATTTTTAAAAAATTGTCACAGTCATGTGCCAAAGTCCTGAAATCTTTCAA TTGTATAGGGACTG | Mutation between -35 and MtlR binding site |
s6726 | AAAAAAGCTAGCTATTTTTAAAAAATTGTCACAGT | Shortening upstream of P mtlA |
s6727 | AAAAAACTTAAGAGAGAATGATGCTTCCCTTTG | Shortening downstream of P mtlA |
s6728 | ATACAATTCTTTCTAAAGAGGACTTTGGCACATG | Mutation between -35 and MtlR binding site |
s6729 | CCTCTTTAGAAAGAATTGTATAGGGACTGTAAGCGT | Mutation between -35 and MtlR binding site |
s6792 | AAAAAAGCTAGCTTTATTTTTAAAAAATTGTCACA | Shortening upstream of P mtlA |
s6793 | AAAAAAGCTAGCTTTTTAAAAAATTGTCACAGTC | Shortening upstream of P mtlA |
s6800 | CGCTTACAGTCCCTATACAAAACTTAGTAAAGAGGACTTTGGCAC | Mutation between -35 and MtlR binding site |
s6801 | CCAAAGTCCTCTTTACTAAGTTTTGTATAGGGACTGTAAGCG | Mutation between -35 and MtlR binding site |
s6829 | AAAAAAGCTAGCTTTAAAAAATTGTCACAGTCAT | Shortening upstream of P mtlA |
s6830 | AAAAAAGCTAGCAAAAATTGTCACAGTCATGTG | Shortening upstream of P mtlA |
s7065 | AAAAAAGCTAGCTTTTTATTTTTAAAAAATTGTCACAGTCATGTGCCAAACAGGACTTTACTTTCAA TTGTATAGGG | Mutation between -35 and MtlR binding site |
s7091 | AAAAAAGCTAGCTTTTTATTTTTAAAAAATTGTCACAGTCATGTGGGTTTGTCCTCTTTACTTTCAA TTGTATA | Mutation between -35 and MtlR binding site |
s7098 | AAAAAAGCTAGCAGCTATTGTAACATAATCGGT | Fusion of P groE -(cre)-UTR PmtlR |
s7189 | CCTTAAAACGCTTACAGCAATTCTTATAATAAAGAATCTCC | Fusion of P groE -(cre PmtlA )-UTR PmtlR |
s7190 | TGCTGTAAGCGTTTTAAGGAAACCTCTCTATATCCTCTA | Fusion of P groE -(cre PmtlA )-UTR PmtlR |
s7191 | CCATAAAACGCTTTCAACAATTCTTATAATAAAGAATCTCC | Fusion of P groE -(cre PmtlR )-UTR PmtlR |
s7192 | TGTTGAAAGCGTTTTATGGAAACCTCTCTATATCCTCTA | Fusion of P groE -(cre PmtlR )-UTR PmtlR |
s7193 | CCTGGTAACGCTTTCAACAATTCTTATAATAAAGAATCTCC | Fusion of P groE -(cre acsA )-UTR PmtlR |
s7194 | TGTTGAAAGCGTTACCAGGAAACCTCTCTATATCCTCTA | Fusion of P groE -(cre acsA )-UTR PmtlR |
s7195 | CCTGTTCACGCTTTCAGCAATTCTTATAATAAAGAATCTCC | Fusion of P groE -(cre mtlA )-UTR PmtlR |
s7196 | TGCTGAAAGCGTGAACAGGAAACCTCTCTATATCCTCTA | Fusion of P groE -(cre mtlA )-UTR PmtlR |
s7237 | GTAGAGGATATAGAGAGGTTTCCCAATTCTTATAATAAAGAATCTCC | Fusion of P groE -UTR PmtlR |
s7238 | GGAGATTCTTTATTATAAGAATTGGGAAACCTCTCTATATCCTCTAC | Fusion of P groE -UTR PmtlR |
Integration vector | Â | |
s5069 | AAAAAAGAATTCGATATCAGATCTACGCGTTAACCCGGGC | Amplification of ermC |
s5070 | AAAAAACAATTGAATCGATTCACAAAAAATAGG | Amplification of ermC |
s5621 | AAAAAAGGCGCCTGGATTACCGTCTCATCG | Amplification of glcT |
s5622 | AAAAAAGGATCCAACCGCTTCCGCCTCATGAA | Amplification of glcT |
s5623 | GTGTTAGTACGCCGTGCTT | Amplification of ptsHI |
s5624 | GTCGCAATCATAGGGAACAT | Amplification of ptsHI |
s5809 | AAAAAAGATATCAACGCCCTTGCCCTTTC | Amplification of ycsN |
s5810 | AAAAAAAGATCTGCATCAGCTGGTAAACTGAT | Amplification of ycsN |
s5812 | AAAAAAAGGCCTAACACAAATGTTGTTTCTGC | Amplification of ydaB |
s5860 | AAAAAAACTAGTACCTGCATGGCACACGT | Amplification of ydaB |
s5866 | AAAAAAGGATCCATAAGAATTGACCTCCTCT | Amplification of glcT-P ptsGHI |
s5867 | AAAAAAGGATCCTAAGGGTGTTAGTACGCCGT | Amplification of ptsHI |
s5918 | AAAGATCTAACCAGGAGCCTTTTTATTTT | Amplification of P mtlA |
s5919 | CGAAATGTAAGGCGATCATATATAAACCCTCCCTGTT | Amplification of P mtlA |
s5920 | AACAGGGAGGGTTTATATATGATCGCCTTACATTTCG | Amplification of mtlD |
s5921 | AAGATATCGACCGTAAACAGCTTCCGTT | Amplification of mtlD |
s5994 | AAACTAGTAAGAAACTTAATCAATAACCGAC | Amplification of ycsA |
s5995 | AAAGGCCTTCTCGATTCCGCTATAATCAG | Amplification of ycsA |
s6067 | CCTGAAAGAAACACCATGCCCGAAC | Amplification of ycnL |
s6068 | AAGATATCGAAAGAAACACCATGCCCGAAC | Amplification of ycnL |
s6079 | AAAAAAACTAGTCTTTGGCACATGACTGTGACA | Amplification of ycnL |
s6080 | AAAGATCTCTTTGGCACATGACTGTGACA | Amplification of ycnL |
s6302 | AAAAAAGAATTCGGTATCTATCTTTTATGCCAA | Amplification of ydjC |
s6303 | AAAAAAGCTAGCTACGTAGTTCTGTCAGCAATC | Amplification of ydjC |
s6304 | AAAAAACTTAAGATCATTGAAGATGTTTCTTGA | Amplification of pspA |
s6305 | AAAAAACATATGCAGCAATTTGATTCGCCGC | Amplification of pspA |
s6344 | AAAAAAGATATCGATCGCCTTACATTTCGGTGC | Amplification of mtlD |
s6345 | AAAAAACATATGTTAAAATGATGGCGTGCAACG | Amplification of mtlD |
s6865 | GCTGACGGCCGGCTCCAGATCT GCA ATC AAG CCT TCA TAT AA | Amplification of mtlR-H342D |
s6866 | TTATATGAAGGCTTGATTGCAGATC TGG AGC CGG CCG TCA GC | Amplification of mtlR-H342D |
s6867 | AAAAAA ACTAGT TTA CAG TAT GTT TTT TTC TTT CAT | Amplification of mtlR-H342D |
s6949 | AAAAAA CCCGGG TAC GAT ATT CCA TAA AAA GC | Amplification of mtlR-H342D |
Primer extension | Â | |
s5959 | Cy5-GCTGCAAGGCGATTAAGTTGG | Hybridized to lacZ |
s5960 | Cy5-CCAGTCACGACGTTGTAAAAC | Hybridized to lacZ |