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Table 1 Strains and plasmids used in this study

From: Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors

Strain or plasmid Genotype or relevant structure Source, reference, or construction
E. coli   
   JM109 recA1, endA1, gyrA96, thi, hsdR17, supE44, relA, λ-, Δ(lac-proAB), [F', traD36, proAB, lacIqZ ΔM15] [69]
B. subtilis   
   3NA spo0A3 [70]
   168 trpC2 Bacillus Genetic Stock Center
   KM12 spo0A3 ΔmtlAF::ermC 3NA transformed with pKAM5
   KM13 spo0A3 ΔmtlAFD::ermC 3NA transformed with pKAM6
   KM15 spo0A3 ΔmtlR::ermC 3NA transformed with pKAM4
   KM37 spo0A3 mtlD::ermC 3NA transformed with pKAM14
   KM39 spo0A3 ΔgutRBPydjE::cat 3NA transformed with pKAM13
   KM40 spo0A3 ΔmtlAFD::ermC ΔgutRBPydjE::cat KM13 transformed with pKAM13
   KM162 spo0A3 ΔmtlR::ermC, hisI', spc KM15 transformed with pHM30
   KM163 spo0A3 mtlR-H342D KM162 transformed with pKAM66
   MW312 spo0A3 (ptsG-pstH')::ermC 3NA transformed with pMW312.2
   MW373 spo0A3 ΔptsG MW312 transformed with pMW373.3
   TQ303 spo0A3 ΔccpA::ermC [5]
   TQ432 trpC2 ptsH-H15A amyE::cat [5]
Plasmids   
   pHM30 hisF, hisI', spc, yvcA, yvcB, bla [37]
   pHM31 hisF, hisI, yvcA, yvcB, bla [37]
   pKAM1 spc, ter- P mtlA (-181/-1)α -lacZ-ter, repA This study
   pKAM4 'ycsN-ermC-ydaB', bla, spc This study
   pKAM5 'ycnL-ermC-P mtlA -mtlD', bla, spc This study
   pKAM6 'ycnL-ermC-ycsA', bla, spc This study
   pKAM9 spc, ter- P mtlA (-161/-1) -lacZ-ter, repA This study
   pKAM12 spc, ter- P mtlA ' (-161/-32)-lacZ-ter, repA This study
   pKAM13 'ydjC-cat-pspA', bla, spc This study
   pKAM14 'mtlD::ermC, bla, spc This study
   pKAM18 spc, ter-P mtlR -lacZ-ter, repA This study
   pKAM27 spc, ter- P mtlA (-161/-32)*[cttta→gaaat] -lacZ-ter, repA This study
   pKAM43 spc, ter- 'P mtlA (-157/-32) -lacZ-ter, repA This study
   pKAM44 spc, ter- P mtlA ' (-161/-52) -lacZ-ter, repA This study
   pKAM45 spc, ter- P mtlA (-161/-32)*[ctttc→gaaag] -lacZ-ter, repA This study
   pKAM48 spc, ter- 'P mtlA (-159/-32) -lacZ-ter, repA This study
   pKAM49 spc, ter- 'P mtlA (-155/-32) -lacZ-ter, repA This study
   pKAM52 spc, ter- P mtlA (-161/-32)*[ttcaa→aagtt] -lacZ-ter, repA This study
   pKAM57 spc, ter- 'P mtlA (-153/-32) -lacZ-ter, repA This study
   pKAM58 spc, ter- 'P mtlA (-149/-32) -lacZ-ter, repA This study
   pKAM59 spc, ter- 'P mtlA (-151/-32) -lacZ-ter, repA This study
   pKAM66 hisF, hisI, mtlR-H342-D, yvcA, yvcB, bla This study
   pKAM84 spc, ter- P mtlA (-161/-32)*[gtcct→cagga] -lacZ-ter, repA This study
   pKAM88 spc, ter- P groE - (cre PmtlA ) - UTR PmtlR -lacZ-ter, repA This study
   pKAM89 spc, ter- P groE - (cre PmtlR ) - UTR PmtlR -lacZ-ter, repA This study
   pKAM90 spc, ter- P groE -(cre acsA ) - UTR PmtlR -lacZ-ter, repA This study
   pKAM91 spc, ter- P groE - (cre mtlA ) - UTR PmtlR -lacZ-ter, repA This study
   pKAM92 spc, ter- P mtlA (-161/-32)*[ccaaa→ggttt] -lacZ-ter, repA This study
   pKAM101 spc, ter- P groE - UTR PmtlR -lacZ-ter, repA This study
   pMW312.2 glcT'-ermC-ptsHI', bla, spc This study
   pMW363.1 ter, spc, manP'-cat-yjdB-yjdA', bla Laboratory Stock
   pMW373.3 'glcT-P ptsGHI -ptsH', bla, spc This study
   pSUN279.2 ter-P manR -manR-P manP -lacZ-ter, repA, ori pUC18 , ori pBS72 , bla, spc [5]
   pSUN338.3 yvcL-ermC-yvcN, bla, spc [5]
   pSUN356.7 manR'-ermC-manA', bla, spc [5]
  1. α The numbers are showing the position of the sequence with respect to mtlA start codon.