Growth of mtl and gut mutants (A) and growth curve of mtlD::ermC mutant (B). (A) Growth of the B. subtilis mtl and gut mutants in minimal medium. Optical density at 600 nm of the strains KM12 (ΔmtlAF), KM13 (ΔmtlAFD), KM15 (ΔmtlR), KM37 (mtlD::ermC), KM39 (ΔgutRBPydjE), KM40 (ΔgutRBPydjE ΔmtlAFD), as well as of B. subtilis 3NA (wild type), was measured after 16 h incubation at 37°C on a rotary shaker with 200 rpm. Minimal medium was supplemented by 1% of either mannitol, glucitol or glucose (control) as the sole carbon source. (B) The toxicity effect of the mannitol and glucitol in KM37. Strain KM37 was grown in LB medium containing 1% glycerol, mannitol, or glucitol and the growth curve was monitored at intervals of 1 h.