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Figure 4 | Microbial Cell Factories

Figure 4

From: Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors

Figure 4

Primer extension. (A) Primer extension of P mtlA in pKAM12. The procedure has been explained in Methods. A, C, G, and T represent the dideoxynucleotide triphosphates used for the pKAM1 sequencing, and the + and - display the primer extension reaction of induced and uninduced sample, respectively. (B) Primer extension of P mtlR in pKAM18 and DNA sequencing reaction.

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