Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440

Wittgens, Andreas; Tiso, Till; Arndt, Torsten T; Wenk, Pamela; Hemmerich, Johannes; Müller, Carsten; Wichmann, Rolf; Küpper, Benjamin; Zwick, Michaela; Wilhelm, Susanne; Hausmann, Rudolf; Syldatk, Christoph; Rosenau, Frank; Blank, Lars M

doi:10.1186/1475-2859-10-80

Microbial Cell Factories

Table 1 Recombinant rhamnolipid production

From: Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440

Organism	Medium	Substrates		Cell Dry Weight	Carbon Yield¹	Maximal Titer	Process Time	RL-Production Rate	Space-Time Yield	Specific RL-Production Rate²	Reference
		Substance	[g/L]	[g_CDW/L]	[Cmol_rhamnolipid/Cmol_substrate]	[g_RL/L]	[h]	[mg_RL/(Lh)]	[mg_RL/(Lh)]	[g/(g_CDW h)]
P. aeruginosa PAO1	MS	Sunflower Oil	250	16.3	0.07	39.00	90	16.67	433.33	0.027	Müller et al. 2010b [20]
P. putida KCTC 1067	MS	Soybean Oil	20	3.2	0.17	7.30	72	3.90	101.39	0.031	Cha et al. 2008 [30]
P. aeruginosa PEER02	MS	Soybean Oil	20	not given	0.04	1.82	96	0.73	18.95	not calculable	Wang et al. 2007 [28]
E. coli HB101	LB	Oleic Acid	4	not given	0.01	0.05	24	0.08	2.18	not calculable	Cabrera-Valladares et al. 2006 [29]
P. putida KT42C1	LB	Glucose	10	2.0	0.23	1.50	42	1.37	35.71	0.018	This study
P. aeruginosa PG201	GS	Glycerol	20	not given	0.17	2.20	168	0.50	13.10	not calculable	Ochsner et al. 1995 [27]
E. coli TnERAB	LB	Glucose	4	not given	0.07	0.18	24	0.28	7.30	not calculable	Wang et al. 2007 [28]
P. aeruginosa PEER02	MS	Glucose	20	not given	0.06	0.79	96	0.31	8.18	not calculable	Wang et al. 2007 [28]
P. putida KT2442	GS	Glycerol	20	1.2	0.05	0.60	25	0.92	24.00	0.020	Ochsner et al. 1995 [27]
E. coli W3110	M9	Glucose	5	not given	0.04	0.12	48	0.10	2.51	not calculable	Cabrera-Valladares et al. 2006 [29]
E. coli TnERAB	MS	Glucose	4	not given	0.03	0.08	24	0.12	3.15	not calculable	Wang et al. 2007 [28]
P. fluorescens ATCC 15453	GS	Glycerol	20	not given	0.02	0.25	168	0.06	1.49	not calculable	Ochsner et al. 1995 [27]
E. coli DH5α	M9	Glucose	5	not given	< 0.01	< 0.02	168	0.00	0.06	not calculable	Ochsner et al. 1995 [27]
P. oleovorans GPo1	GS	Glycerol	20	not given	0.00	< 0.02	168	0.00	0.06	not calculable	Ochsner et al. 1995 [27]

A comprehensive overview of production performance of recombinant strains is presented. For comparison, wild type P. aeruginosa PAO1 is listed in line 1. The next three lines show data from experiments with hydrophobic substrates in contrast to the remaining entries. This data is based on hydrophilic substrate experiments.
¹) For the calculation of yields during production on complex media, rhamnolipids were assumed to be synthesized from the used carbon source, while media compounds were utilized for cell growth. ²) The specific rhamnolipid production rate was calculated as average over the whole fermentation time. LB, Lysogeny broth medium; MS, mineral salt medium; GS, nitrogen-limited minimal medium.

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ISSN: 1475-2859

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