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Table 1 Effect of metal ions on the stability of alkaline α-amylase

From: Heterologous expression, biochemical characterization, and overproduction of alkaline α-amylase from Bacillus alcalophilus in Bacillus subtilis

Metal ions

Relative activity (%)b

 

1 mM

10 mM

CKa

100

100

Na+

110 ± 2

96 ± 2

Co2+

97 ± 2

96 ± 2

Zn2+

80 ± 1

85 ± 1

Ca2+

102 ± 2

85 ± 3

K+

97 ± 2

100 ± 2

Mn2+

37 ± 1

27 ± 3

Mg2+

86 ± 2

84 ± 2

Fe2+

74 ± 2

84 ± 2

Cu2+

81 ± 1

88 ± 1

Fe3+

90 ± 2

86 ± 1

  1. a The relative activity was calculated on the basis of the activity (CK) obtained in glycine/NaOH buffer (pH 10.0) without the addition of any metal ions.
  2. b Values represent means ± SD (n = 3) relative to the untreated control samples.
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Microbial Cell Factories

ISSN: 1475-2859

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