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Figure 1 | Microbial Cell Factories

Figure 1

From: Real-Time monitoring of intracellular wax ester metabolism

Figure 1

Operating principle of the wax ester monitoring system in engineered A. baylyi ADP1. A) The bacterial luciferace LuxAB utilizes the specific intermediate in wax ester synthesis route, fatty aldehyde, as a substrate producing light and fatty acid molecule which is returned to the carbon cycle. Enzymatic steps involved in the natural lipid metabolism of ADP1 are as follows: 1 - Fatty acyl-CoA is reduced to corresponding fatty aldehyde by the fatty acyl-CoA reductase (acr1); 2 - Fatty aldehyde is reduced to corresponding fatty alcohol by an aldehyde reductase; 3 - Fatty alcohol is esterified with fatty acyl-CoA by WS/DGAT (wax-dgaT) forming a wax ester; 4 - Free fatty acid is recycled by acyl-CoA synthetases. B) A schematic presentation of the engineered strains Wab+ and Wab- against the wild type genotype. The gene cassette iluxAB_Cmr containing the genes luxAB and chloramphenicol resistance under constitutive promoter was used to replace a neutral gene poxB. In Wab-, acr1 is replaced with a gene cassette Kanr/tdk [16].

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