Figure 1

Anti-biofilm activity of supernatants from different strains (SP1-SP10) associated with Spongia officinalis. Biofilms of Escherichia coli PHL628 were allowed to develop in the presence of supernatants (3% v/v) from marine sponge-associated isolates in 96 well microtiter well. The plate was incubated at 30°C for 36 h, followed by crystal violet staining and spectrophotometric absorbance measurements (OD₅₇₀). The absorbance was used to calculate the "biofilm formation" on the y axis. × axis represents cell free supernatants from different Spongia officinalis isolates. The 100% is represented by E. coli PHL628 produced biofilm.