Figure 6
From: Optimisation of surface expression using the AIDA autotransporter
Batch cultivation with repeated glucose addition. (A) Cell growth measured as optical density (filled squares) and dry weight (open squares), and surface expression measured by flow cytometry (circles) as a function of cultivation time. Growth curves are fitted to exponential functions to approximate the growth rates (optical density and dry weight). (B) Growth rate (diamonds), acetic acid concentration in the growth medium (triangles) and dissolved oxygen tension (DOT, line) as a function of time. Downward pointing arrows indicate time points for the five additions of glucose. The leftmost upwards pointing arrow indicates the starting point for manual DOT regulation by increasing of the stirring or the air-flow, and the rightmost upwards pointing arrow indicates the point at which the maximum stirrer speed was reached.