Figure 4
From: Optimisation of surface expression using the AIDA autotransporter
Effects of growth medium. (A) Comparison of surface expression of protein Z in minimal medium with glucose (grey), LB medium (white) and LB medium with glucose (black). Samples were taken at OD600 = 0.5 and 3. (B) Consumption of amino acids and production of NH3 in LB medium (filled symbols) and LB medium with glucose (open symbols) as a function of cultivation time. Legend: Serine (circles), aspartate (squares), threonine (upwards pointing triangle), proline (downwards pointing triangle) and NH3 (diamonds). Arrow: depletion of the first amino acid (serine) in the LB cultures.