Table 3 Primers used for gene deletion and verification in this study
Primer Name | Nucleotide Sequence | Characterization |
|---|---|---|
tyrB-KO-F | 5'-TTTAACCACCTGCCCGTAAACCTGGAGAACCATCGCGTG ATTCCGGGGATCCGTCGACC- 3' | Deletion primer |
tyrB-KO-R | 5'-ACTGCAGGCTGGGTAGCTCCAGCCTGCTTTCCTGCATTA TGTAGGCTGGAGCTGCTTC- 3' | Deletion primer |
tyrB-V-F | 5'- CTGTTGCTAATTGCCGTTCG - 3' | Verification primer |
tyrB-V-R | 5'- CACGTAGAACGATGGCATCA - 3' | Verification primer |
aspC-KO-F | 5'-CGGACTTCCCTTCTGTAACCATAATGGAACCTCGTCATG ATTCCGGGGATCCGTCGACC- 3' | Deletion primer |
aspC-KO-R | 5'-AGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGC TTA TGTAGGCTGGAGCTGCTTC- 3' | Deletion primer |
aspC-V-F | 5' - CCTGCGTTTTCATCAGTAATAGTTGG - 3' | Verification primer |
aspC-V-R | 5' - CCTTATCCGGCCTACAAAATCG - 3' | Verification primer |
tyrA-V-F | 5' - TATCCGTAACCGATGCCTGC - 3' | Verification primer |
tyrA-V-R | 5' - GGGAAATCACCCGTTCAATG - 3' | Verification primer |
trpE-V-F | 5' - CGTACTGAAAGGTTGGTGGCG - 3' | Verification primer |
trpE-V-R | 5' - AGGAGAAAGCATCAGCACCG - 3' | Verification primer |