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Table 1 The numbers of proper strains among all single, double, triple, and quadruple deletion mutants generated from the backbone models for E. coli (iBKEco52) and S. cerevisiae (iBKSce50, iBKSce50Δmit, and iBKSce50+7)

From: Engineering strategy of yeast metabolism for higher alcohol production

Target higher alcohols

Number of proper strains

Total (single/double/triple/quadruple deletion mutants)

 

iBKEco52

iBKSce50

iBKSce50Δmit

iBKSce50+7

1-propanol from OAA

458

(0/11/85/362)

40

(2/16/14/8)

108

(4/27/50/27)

467

(4/23/128/312)

1-Butanol from AcCoA

347

(1/17/87/242)

1

(1/0/0/0)

49

(2/7/17/23)

192

(33/22/44/93)

1-Butanol from OAA

501

(0/10/89/402)

71

(0/10/30/31)

120

(3/25/59/33)

373

(2/17/116/238)

Isobutanol from PYR

501

(1/14/83/403)

38

(2/15/21/0)

85

(4/25/30/26)

276

(2/18/81/175)

3-Methyl-1-butanol from PYR

208

(2/13/51/142)

46

(1/15/21/9)

98

(4/25/46/23)

94

(2/5/33/54)

Isopentenol from AcCoA

330

(0/5/55/270)

0

(0/0/0/0)

104

(0/12/51/41)

166

(2/10/54/100)

  1. The "proper" strains were defined as viable target-producing strains in which all deletions contributed to the improvement of product yields. The growth rates and product yields were determined by FBA-based metabolic simulations. iBKSce50Δmit is an hypothetical S. cerevisiae model derived by merging the cytosolic and mitochondrial networks into one compartment. iBKSce50+7 is a model of iBKSce50 expanded by the addition of 7 E. coli reactions.