Aim of primers | Name | Sequence (5'→3') |
---|---|---|
For csnN106 coding region cloning* | FwcsnN106 | CCGGAGACCCGCATGC CCCGGAC |
 | RvcsnN106 | CGGTGCGCCAAGCTT GCGTTCGG |
For Pr-WT cloning* | FwPr-WT | GTCTGCGCGGATCC TGACGGCCC |
 | RvPr-WT | GTCCGGGGCATGC GGGTCTCCGG |
PCR-directed mutagenesis for Pr-Pa cloning** | SEQ.1 | ACAACTTCGTCGCGCACATCCA |
 | Rw1Pr-Pa | ATGAGGAGAGTTCGGACAGTTTC |
 | Fw2Pr-Pa | GAAACTG TCCG AACTCTCCTCAT |
 | RvcsnN106 | TGAGGTCGAAGTTCTTGGCGTT |
Verification of pHM8a derivatives integration into hosts | Fwgenom | CCTGAGAGGCCGGTGAGGAG |
 | RvcsnN106 | TGAGGTCGAAGTTCTTGGCGTT |
Presence verification of pFDES derivatives into hosts | SEQ.1 | ACAACTTCGTCGCGCACATCCA |
 | T7 promoter | TTAATACGACTCACTATAGGG |
For Primer extension | PE-csnN106 | TGGGGTGCTTGAGACGCAT |