Figure 1From: Modification of genetic regulation of a heterologous chitosanase gene in Streptomyces lividans TK24 leads to chitosanase production in the absence of chitosanPrimer extension analysis of csnN106 transcripts. The apparent 5' terminus for the csnN106 transcript was identified by annealing a radiolabeled primer complementary to the mRNA of csnN106 and extension with reverse transcriptase. 40 μg of total RNA, from GlcN-chitosan oligomers induced S. lividans TK24(pHPr-WT), were used for extension reaction. The same primer was used for DNA sequencing reactions with the pHPr-WT plasmid. (→): primer extension product; (*): apparent transcription start site. Vertical arrows: palindromic sequence.Back to article page