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Table 3 Identification by MALDI-MS and ESI-LC MS/MS of proteins secreted by T. reesei Rut C-30 grown in SHMM and LBSM

From: Enzyme production by filamentous fungi: analysis of the secretome of Trichoderma reesei grown on unconventional carbon source

Num

ProtIDa

Protein name

Precursor mass (kDa)

pIb

Mascot scorec

Peptides

matched

%Sequence

coverage

Sigd

1

121127

β-Xylosidase (BXL1)

87.1

5.9

110 *

14

29.0

Y

2

121127

β-Xylosidase (BXL1)

87.1

5.8

134 *

18

31.5

Y

3

121127

β-Xylosidase (BXL1)

87.1

5.7

133 *

16

26.0

Y

4

121127

β-Xylosidase (BXL1)

87.1

5.6

127 *

17

29.5

Y

5

121127

β-Xylosidase (BXL1)

87.1

5.5

102 *

16

28.6

Y

6

123989

Cellobiohydrolase I (Cel7A)

54.1

4.4

64 *

6

16.7

Y

7

123989

Cellobiohydrolase I (Cel7A)

54.1

4.6

62 *

6

16.7

Y

8

123989

Cellobiohydrolase I (Cel7A)

55.4

4.5

73*

5

13

Y

9

123989

Cellobiohydrolase I (Cel7A)

55.4

4.5

73*

5

13

Y

10

123989

Cellobiohydrolase I (Cel7A)

55.4

4.4

73*

5

13

Y

11

81598

Chitinase (CHI)

44.5

5.3

464#

9

18

Y

12

107776

D-Xylose reductase (XYL1)

36.5

6.1

99 *

10

44.4

N

13

107776

D-Xylose reductase (XYL1)

36.5

6.1

606#

12

39

N

14

121661

Aldose 1-epimerase 2 (AEP2)

37.1

5.3

194 *

18

62.9

N

15

121661

Aldose 1-epimerase 2 (AEP2)

37.1

5.4

136 *

17

56.4

N

16

22426

D-xylose dehydrogenase (XYL2)

41.5

6.7

241 *

21

67

Y

17

77579

Aspartyl protease (ASPP)

42.4

4.5

118 *

11

25

Y

18

58493

Cytochrome c oxidase, subunit Vb (CytC)

16.6

5.2

175#

4

20

N

19

51365

Subtilisin kexin isozyme-1 (SKI1)

93.3

4.6

111 *

10

17.9

Y

20

51365

Subtilisin kexin isozyme-1 (SKI1)

93.3

4.7

143 *

12

22.7

Y

21

51365

Subtilisin kexin isozyme-1 (SKI1)

93.3

4.8

142 *

12

21.2

Y

22

120911

Predicted arabinofuranosidase (ABFII)

36.3

6.7

379#

14

31

Y

23

42919

Ubiquitin fusion protein (Ub)

8.3

4.5

89#

3

41

N

24

123902

Translation elongation factor 1 (EF1)

45.9

4.6

109 *

14

37

Y

25

73967

Actin depolymerizing factor (ADF)

17.4

6.0

108 *

7

53

N

26

81586

Xylanase II (XYN II)

18.8

6.5

104 *

8

47

Y

27

123029

Copper/Zinc superoxide dismutase (SOD1)

15.8

6.1

80 *

5

55

Y

28

23200

Fructose 1,6-bisphosphate aldolase (FPA)

39.4

6.0

142 *

13

45

N

29

23200

Fructose 1,6-bisphosphate aldolase (FPA)

39.4

5.9

99*

16

50.8

N

30

2433

7alpha-Cephem-methoxylase (SME)

32.1

5.4

149 *

20

84

N

31

120568

Enolase (ENO)

47.3

5.2

143 *

15

43

N

32

72567

Cellobiohydrolase II (Cel6A)

50.3

5.2

100 *

9

27

Y

33

72567

Cellobiohydrolase II (Cel6A)

50.3

5.4

107*

10

27

Y

34

73631

Isoamyl alcohol oxidase (IACOX)

60.9

5.2

169 *

17

32

Y

35

123026

Transaldolase (TSD)

35.6

5.1

78 *

9

29

N

36

73564

20S proteasome, subunit (PSA3)

31.7

5.1

99 *

10

34

N

37

74223

Xylanase I (XYN I)

24.6

4.4

86#

5

49.6

Y

38

78683

Aldehyde dehydrogenase (ADH)

53.7

5.5

250#

6

14

N

39

78683

Aldehyde dehydrogenase (ADH)

53.7

5.5

334#

5

12

N

40

73842

Predicted beta-galactosidase (BGA1)

97.5

5.1

85 *

10

39

Y

41

123114

Similar to Hsp90

78.5

4.7

87#

2

5

Y

42

105808

Peptidase M (PDM)

33.9

4.9

79 *

5

22

Y

43

107639

Predicted epimerase (EPM)

35.1

6.2

183 *

17

72

Y

44

76672

Beta-glucosidase (BGL1)

78.7

6.4

196 *

20

49

Y

45

109282

Flavonol/cinnamoyl-CoA reductase (FCR)

39.3

4.5

90 *

9

33.0

N

46

22426

Dimeric dihydrodiol dehydrogenase (DDH)

41.5

6.5

241 *

21

67

Y

47

123071

ATP synthase beta chain (ATP2)

54.8

5.3

212 *

23

62

N

48

79324

Flavonol/cinnamoyl-CoA reductase (FCR)

38.2

6.1

137 *

15

42.9

N

49

79324

Flavonol/cinnamoyl-CoA reductase (FCR)

38.2

6.2

156 *

12

35.2

N

50

122081

Endoglucanase I (Cel7B)

48.2

4.7

402#

13

16.3

Y

51

122081

Endoglucanase I (Cel7B)

48.2

4.8

106#

4

9.2

Y

52

120312

Endoglucanase II (Cel5A)

44.1

5.8

219#

5

18.4

Y

  1. a From T. reesei genome database: http://genome.jgi-psf.org/Trire2/Trire2.home.html.
  2. b p Is determined by 2-DE.
  3. c Analyzed by MALDI-MS (*) or LC-MS/MS (#)
  4. d With (Y) or without (N) predicted signal sequence for exporting.